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fluorescence labeled secondary antibody  (Yeasen Biotechnology)

 
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    Yeasen Biotechnology fluorescence labeled secondary antibody
    MOTS-c exposure attenuates oxidative stress in HUVECs. (A) Relative intensity of DCFH-DA staining. (B) Cellular SOD activity. (C) Cellular MDA content. (D) Representative images of MitoSOX staining. Scale bar, 10 μ m. (E) Representative images of JC-1 staining. Scale bar, 50 μ m. (F) Representative immunofluorescence images of Nrf2 in HUVECs. Scale bar, 5 μ m. (G) Relative nuclear to cytoplasm <t>fluorescence</t> ratio. Results are representative of three independent experiments. Data are expressed as the mean ± SD, n=4. *** P<0.001, # P<0.05 vs. PBS under hypoxic conditions, ## P<0.01 vs. PBS under hypoxic conditions; ### P<0.001 vs. PBS under hypoxic conditions. SOD, superoxide dismutase; MDA, malondialdehyde; DCFH-DA,2'7'-dichlorodihydro fluorescein diacetate ; Nrf2, nuclear factor erythroid 2-related factor 2.
    Fluorescence Labeled Secondary Antibody, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+labeled+secondary+antibody/pmc12651125-117-10-19?v=Yeasen+Biotechnology
    Average 86 stars, based on 1 article reviews
    fluorescence labeled secondary antibody - by Bioz Stars, 2026-08
    86/100 stars

    Images

    1) Product Images from "MOTS-c protects against placental injury via Nrf2 activation in hypoxia-induced intrauterine growth restriction mice"

    Article Title: MOTS-c protects against placental injury via Nrf2 activation in hypoxia-induced intrauterine growth restriction mice

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2025.5697

    MOTS-c exposure attenuates oxidative stress in HUVECs. (A) Relative intensity of DCFH-DA staining. (B) Cellular SOD activity. (C) Cellular MDA content. (D) Representative images of MitoSOX staining. Scale bar, 10 μ m. (E) Representative images of JC-1 staining. Scale bar, 50 μ m. (F) Representative immunofluorescence images of Nrf2 in HUVECs. Scale bar, 5 μ m. (G) Relative nuclear to cytoplasm fluorescence ratio. Results are representative of three independent experiments. Data are expressed as the mean ± SD, n=4. *** P<0.001, # P<0.05 vs. PBS under hypoxic conditions, ## P<0.01 vs. PBS under hypoxic conditions; ### P<0.001 vs. PBS under hypoxic conditions. SOD, superoxide dismutase; MDA, malondialdehyde; DCFH-DA,2'7'-dichlorodihydro fluorescein diacetate ; Nrf2, nuclear factor erythroid 2-related factor 2.
    Figure Legend Snippet: MOTS-c exposure attenuates oxidative stress in HUVECs. (A) Relative intensity of DCFH-DA staining. (B) Cellular SOD activity. (C) Cellular MDA content. (D) Representative images of MitoSOX staining. Scale bar, 10 μ m. (E) Representative images of JC-1 staining. Scale bar, 50 μ m. (F) Representative immunofluorescence images of Nrf2 in HUVECs. Scale bar, 5 μ m. (G) Relative nuclear to cytoplasm fluorescence ratio. Results are representative of three independent experiments. Data are expressed as the mean ± SD, n=4. *** P<0.001, # P<0.05 vs. PBS under hypoxic conditions, ## P<0.01 vs. PBS under hypoxic conditions; ### P<0.001 vs. PBS under hypoxic conditions. SOD, superoxide dismutase; MDA, malondialdehyde; DCFH-DA,2'7'-dichlorodihydro fluorescein diacetate ; Nrf2, nuclear factor erythroid 2-related factor 2.

    Techniques Used: Staining, Activity Assay, Immunofluorescence, Fluorescence



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    MOTS-c exposure attenuates oxidative stress in HUVECs. (A) Relative intensity of DCFH-DA staining. (B) Cellular SOD activity. (C) Cellular MDA content. (D) Representative images of MitoSOX staining. Scale bar, 10 μ m. (E) Representative images of JC-1 staining. Scale bar, 50 μ m. (F) Representative immunofluorescence images of Nrf2 in HUVECs. Scale bar, 5 μ m. (G) Relative nuclear to cytoplasm <t>fluorescence</t> ratio. Results are representative of three independent experiments. Data are expressed as the mean ± SD, n=4. *** P<0.001, # P<0.05 vs. PBS under hypoxic conditions, ## P<0.01 vs. PBS under hypoxic conditions; ### P<0.001 vs. PBS under hypoxic conditions. SOD, superoxide dismutase; MDA, malondialdehyde; DCFH-DA,2'7'-dichlorodihydro fluorescein diacetate ; Nrf2, nuclear factor erythroid 2-related factor 2.
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    Image Search Results


    MOTS-c exposure attenuates oxidative stress in HUVECs. (A) Relative intensity of DCFH-DA staining. (B) Cellular SOD activity. (C) Cellular MDA content. (D) Representative images of MitoSOX staining. Scale bar, 10 μ m. (E) Representative images of JC-1 staining. Scale bar, 50 μ m. (F) Representative immunofluorescence images of Nrf2 in HUVECs. Scale bar, 5 μ m. (G) Relative nuclear to cytoplasm fluorescence ratio. Results are representative of three independent experiments. Data are expressed as the mean ± SD, n=4. *** P<0.001, # P<0.05 vs. PBS under hypoxic conditions, ## P<0.01 vs. PBS under hypoxic conditions; ### P<0.001 vs. PBS under hypoxic conditions. SOD, superoxide dismutase; MDA, malondialdehyde; DCFH-DA,2'7'-dichlorodihydro fluorescein diacetate ; Nrf2, nuclear factor erythroid 2-related factor 2.

    Journal: International Journal of Molecular Medicine

    Article Title: MOTS-c protects against placental injury via Nrf2 activation in hypoxia-induced intrauterine growth restriction mice

    doi: 10.3892/ijmm.2025.5697

    Figure Lengend Snippet: MOTS-c exposure attenuates oxidative stress in HUVECs. (A) Relative intensity of DCFH-DA staining. (B) Cellular SOD activity. (C) Cellular MDA content. (D) Representative images of MitoSOX staining. Scale bar, 10 μ m. (E) Representative images of JC-1 staining. Scale bar, 50 μ m. (F) Representative immunofluorescence images of Nrf2 in HUVECs. Scale bar, 5 μ m. (G) Relative nuclear to cytoplasm fluorescence ratio. Results are representative of three independent experiments. Data are expressed as the mean ± SD, n=4. *** P<0.001, # P<0.05 vs. PBS under hypoxic conditions, ## P<0.01 vs. PBS under hypoxic conditions; ### P<0.001 vs. PBS under hypoxic conditions. SOD, superoxide dismutase; MDA, malondialdehyde; DCFH-DA,2'7'-dichlorodihydro fluorescein diacetate ; Nrf2, nuclear factor erythroid 2-related factor 2.

    Article Snippet: At room temperature, the samples were co-incubated with the corresponding fluorescence labeled secondary antibody (1:100; cat. no. 33106ES60; Shanghai Yeasen Biotechnology Co., Ltd.) for 60 min, and freshly prepared DAB (cat. no. #CW20695; CWBIO) staining was then performed.

    Techniques: Staining, Activity Assay, Immunofluorescence, Fluorescence